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Assay Method Information |
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| | Kinase Inhibition Assay |
| Description: | Compounds were evaluated for their ability to prevent phosphorylation of a model glutamate-tyrosine copolymer substrate by isolated human FGFR-1, mouse PDGF-beta receptor (PDGFR), or avian c-Src tyrosine kinase (all full length enzymes). IC50 is defined as the concentration of inhibitor that reduces by 50% the level of gamma-32P incorporated into the copolymer substrate. |
| Affinity data for this assay | |
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