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| | PTP1B Activity Assay |
| Description: | Assays for the reversible inhibition of PTP1B (72 nM) contained the test compound(2a, 2b, 5a, or 5b) in Bis-Tris (50 mM), NaCl (100 mM), EDTA (2 mM), p-nitrophenyl phosphate (p-NPP, 0.4 mM), DTT (5 mM), Tween 80 [0.05% (v/v)], and DMSO [2% (v/v)] at pH 7.0. The test compounds were introduced into the solutions as stock solutions dissolved in DMSO. After 20−30 s, the amount of PTP1B activity in the assays was assessed by measuring the increase in absorbance at 410 nm as a function of time resulting from the enzyme-mediated conversion of p-NPP to p-nitrophenolate. |
| Affinity data for this assay | |
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