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Assay Method Information |
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| | Equilibrium Binding/Displacement Assay with Fluorescence Anisotropy |
| Description: | All measurements were performed in HEPES (50 mm, pH 7.5) containing NaCl (150 mm), Tween-20 (0.005%) and DTT (5 mm) according to a previously published protocol.[Vaasa et al., Anal. Biochem., 385:85-93] The final volume of the assay mixture was 20 mL. |
| Affinity data for this assay | |
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