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| | LSD1 Screening Assay |
| Description: | The LSD1 screening biochemical assay was performed by Shanghai ChemPartner Co. Ltd and the detailed protocol was shown as followed. The AlphaLISA assay of LSD1 (BPS Bioscience) was performed in modified Tris buffer (pH 7.5). Briefly, the compoundsin DMSO were transferred to assay plate by Echo 550, followed by pre-incubating with enzyme (final concentration is 5 nM) for 15 min at room temperature. The initiation of reactions were added H3K4me2 peptide solution (final concentration is 100 nM) to the reaction mixtures and then incubated for 60 min at roomtemperature. The reactions were terminated by the addition of the acceptor and donor beads mix (PerkinElmer) and incubated for 60 min at room temperature, and finally read on EnSpire (PerkinElmer) in Alpha Mode. |
| Affinity data for this assay | |
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