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Assay Method Information

Assay Name:  In Vitro AChE/BChE Inhibition Assay
Description:  The test was run as described in the following procedure: stock solution of the test compounds was diluted in phosphate buffer pH 7.4 to give final concentrationsbetween: 0.1 nM and 2400 nM. AChE and BChE were dissolved in phosphate buffer pH 7.4 to give 2 U/ml and 4 U/ml, respectively. Furthermore, the acetylthiocholine (ATCh) iodide solutions 1 mM and 2 mM for AChE assay and BChE assay were prepared, respectively. The test medium contained DTNB solution 0.4 mg/ml (76 μl), AChE solution 2 U/ml or BChE solution 4 U/ml (10 μl) respectively, various concentrations of the tested compounds (14 μl) and ATCh iodide solutions 1 mM or 2 mM (40 μl) for AChE or BChE, respectively. The medium was mixed and absorption at412 nm was measured after 10 minutes for AChE and after 8 minutes for BChE at room temperature. Each experiment was performed in triplicate. For the reference value, 14 μl of the test compound solutions were replaced by phosphate buffer pH 7.4.
Affinity data for this assay
 

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Last update November 1, 2007
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