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Assay Method Information |
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| | Fluorescence Competition Assay |
| Description: | The method entails two steps [Lin et al., Biochemistry, 38:185-190]. In the first step, the Kd for association of FABP5 with the fluorescent lipid probe anilinonaphtalene-8-sulfonic acid (ANS) was measured by fluorescence titrations. Kd values for nonfluorescent ligands were then measured by monitoring their ability to compete with the probe for binding. FABP5 was precomplexed with ANS and titrated with ligands. Ligand binding was followed by monitoring the decrease in fluorescence upon displacement of the probe from the protein. |
| Affinity data for this assay | |
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