Assay Method Information |
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| CDKs Assay |
Description: | The enzyme was assayed with substrate GST- retinoblastoma in the presence of 25 uM ATP/[gamma-32P] ATP. IC50 is the inhibitor concentration, which inhibits 50% of enzyme activity that catalyzes the transfer of the terminal phosphate from [gamma-32P] labeled ATP to GST- retinoblastoma. Use of 32P-labeled ATP allows this transfer to be monitored by scintillation counting. |
Affinity data for this assay | |
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