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Assay Method Information

Assay Name:  α-Glucosidase Inhibition Assay
Description:  For α-glucosidase inhibition assay, α-glucosidase enzyme (Cat No. 5003-1KU Type I; isolated from Saccharomyces cereviciae) was used. Acarbose was the reference standard and Pierre's methodology was employed [Pierre et al., J. Clin. Chem., 24:208-211]. First absorbance was taken at 400 nm after the 10 min incubation of a mixture of phosphate buffer (70 mL, pH 6.8), test compound (10 mL, 0.5 mM) and enzyme (10 mL, 0.0234 units, Sigma Inc.). Later, 10 mL of p-nitrophenyl-α-D-glucopyranoside (0.5 mM, code No. N1377 from Sigma Inc) was added. After 30 min incubation at 37 °C, the absorbance was retaken at 400 nm.
Affinity data for this assay
 

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Last update November 1, 2007
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