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Assay Method Information

Assay Name:  MT Kinetic Assay
Description:  The assay was performed as previously described with slight modifications [Chen et al., J. Agric. Food Chem., 50:4108-12]. The reaction medium was 1 mL in 50 mM phosphate buffer (pH 6.8). The final concentration of mushroom tyrosinase was 26.07 μg/mL for cresolase activity and 6.52 μg/mL for catecholase activity. Freshly prepared enzyme, substrate, and dithiocarbamates I and II were used in this work. The reaction was carried out under constant temperatures of 27 and 37C. Substrate addition followed incubation of the enzyme with different concentrations of synthetic inhibitors.
Affinity data for this assay
 

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Last update November 1, 2007
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