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Assay Method Information

Assay Name:  GST Assay
Description:  A range of natural products were screened for inhibition of PfGST by GST assay with CDNB as a substrate, using a 96-well SpectraMax 340 microplate spectrophotometer equipped with a kinetics mode (Molecular Devices, California). The final concentration of CDNB was 0.5 mM and of GSH was 1mM. Ethacrynic acid (ETA) and haemin were used as positive controls. The incubation temperature was 30C. The final concentration of organic solvent in the inhibition assays was 2.5%. Initially the natural plant compounds were tested at arbitrary concentrations of 33 μM and 100 μM.
Affinity data for this assay
 

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Last update November 1, 2007
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