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| | Enzyme Kinetic Assay |
| Description: | The inhibition of the HLE was studied at 25°C by continuously monitoring the absorbance at 410 nm for 20 min of a solution prepared by mixing 10 μL of HLE stock solution (2 μM in 0.05M acetate buffer, pH 5.5) with a solution containing 10 μL of 5 in DMSO, 20 μL of MeOSuc-Ala-Ala-Pro-Val-p-NA (50 mM in DMSO) and 960 μL of 0.1M pH 7.2 HEPES buffer. Control assays, in which the inhibitor was omitted, ran linearly. |
| Affinity data for this assay | |
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