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Assay Method Information

Assay Name:  CA Activity Assay
Description:  CA activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a period of 3 min at 25C using a spectrophotometer (CHEBIOS UV-VIS) according to the method described by Verpoorte et al [Verpoorte et al., J. Biol. Chem., 242:4221-4229]. The enzymatic reaction, in a total volume of 3.0 mL, contained 1.4 mL 0.05 M Tris-SO4 buffer (pH 7.4), 1 mL 3 mM 4-NPA, 0.5 mL H2O and 0.1 mL enzyme solution. A reference measurement was obtained by preparing the same cuvette withoutenzyme solution.
Affinity data for this assay
 

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Last update November 1, 2007
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