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| | Cell-Based Enzyme Inhibition Assay (ELISA) |
| Description: | Detection of the effect of compounds on p-MEK1/2, t-ERK1/2, pERK1/2, p-Rsk1 and p-Elk-1 activity in B16-F10 cells was performed using ELISA kits (Invitrogen)and strictly according to the manufacturer's instructions. Cells were pre-treated with 30 nM PMA (Sigma) in the absence or presence of these compounds. |
| Affinity data for this assay | |
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