Assay Method Information |
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| Esterase Activity Assay |
Description: | CA activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a period of 3 min at 25°C using a spectrophotometer (CHEBIOS UV-Vis) according to the method described by Verpoorte et al. [Verpoorte et al., J. Biol. Chem., 242:4221-4229]. The enzymatic reaction contained 1.4 mL 0.05 M Tris-SO4 buffer(pH 7.4), 1 mL 3 mM 4-nitrophenylacetate, 0.5 mL H2O and 0.1 mL enzyme solution (total volume, 3.0 mL). A reference measurement was obtained by preparing themixture without the enzyme solution. All measurements were made in triplicate. |
Affinity data for this assay | |
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