Assay Method Information |
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| Esterase Activity Assay |
Description: | Carbonic anhydrase activity was assayed by following the change in absorbance at 348 nm of 4-nitrophenylacetate (NPA) to 4-nitrophenylate ion over a period of 3 min at 25°C using a spectrophotometer (CHEBIOS UV-VIS) according to the method described by Verpoorte et al. [Verpoorte et al., J. Biol. Chem., 242:4221-4229].The inhibitory effects of compounds 1-14 and AZA were examined. All compounds were tested in triplicate at each concentration used. |
Affinity data for this assay | |
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