Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Cannabinoid Receptor Binding Assay |
| Description: | Membranes from HEK-293 cells transfected with the human recombinant CB1 receptor(Bmax=2.5pmol/mg protein) and human recombinant CB2 receptor (B max=4.7 pmol/mgprotein) were incubated with [3H]-CP-55,940 (0.14 nM / Kd = 0.18 nM and 0.084nM / Kd =0.31 nM respectively for CB1 and CB2 receptor) as the high affinity ligand and displaced with 10 μM WIN 55212-2 as the heterologous competitor for non specific binding (Ki values 9.2 nM and 2.1 nM respectively for CB1 and CB2 receptor). All compounds were tested following the procedure described by the manufacturer (Perkin Elmer, Italy). Displacement curves were generated by incubating drugs with [3H]-CP-55,940 for 90 minutes at 30 C. Ki values were calculated by applying the Cheng-Prusoff equation to the IC50 values (obtained by GraghPad) for the displacement of the bound radioligand by increasing concentrations of the testcompound. Data are meansąSEM of at least n=3 experiments. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||