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| | In Vitro AChE and BuChE Inhibition Assays |
| Description: | All the assays were under 0.1 M KH2PO4/K2HPO4 buffer, pH 8.0, using a Shimadzu 2450 Spectrophotometer. Enzyme solutions were prepared to give 2 units/mL in 2 mL aliquots. The assay medium contained phosphate buffer, pH 8.0 (1 mL), 50 μL of 0.01 M DTNB, 10 μL of enzyme and 50 μL of 0.01 M substrate (acetylthiocholine chloride). The substrate was added to the assay medium containing enzyme, buffer and DTNB with inhibitor after 15 min of incubation time. The activity was determined by measuring the increase in absorbance at 412 nm at 1 min intervals at 37°C. |
| Affinity data for this assay | |
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