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Assay Method Information |
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| | Thermal Shift Assay (TSA) |
| Description: | The thermal shift assay (TSA) measurements were performed in a Corbett Rotor-Gene 6000 (QIAGEN Rotor-Gene Q, Sydney, Australia) instrument using the blue channel (excitation 365±20, detection 460±15 nm). Samples contained 10 μM protein, 0-200 μM compound, 50 mM solvatochromic dye ANS (8-anilino-1-naphthalene sulfonate) and 50mM phosphate buffer containing 100 mM NaCl at pH 7.0, with the final DMSO concentration at 2%. The applied heating rate was 1 °C/min. Data analysis was performed as previously described [Kazlauska et al., PLoS ONE, 7:e36899]. |
| Affinity data for this assay | |
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