BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Enzyme Immunoassay (EIA) Assay
Description:  I. Assay Solutions a. Preparation of 0.1M K2HPO4/KH2PO4 buffer (pH 7.4) Prepare 0.1 M KH2PO4 from 1M KH2PO4 (Sigma, Cat# P-8709) Prepare 0.1 M K2HPO4 from powder of K2HPO4 (Fisher, BP363-500) Mix 0.1 M K2HPO4 with 0.1 M KH2PO4 to adjust pH to 7.4. b. Preparation of 0.5% γ-globulin Add 0.1 g of γ-globulin (Sigma, Cat# G-5009) to 20 mL 0.1 M K2HPO4/KH2PO4 buffer (pH 7.4) and make 1-mL/vial aliquots and store in −80 C. c. Preparation of 100 mM GSH Add 307 mg of GSH (Sigma, Cat# G-6529) to 10 mL 0.1 M K2HPO4/KH2PO4 buffer (pH 7.4) and store at −80 C. d. Preparation of Reaction buffer: 198 mL of 0.1M K2HPO4/KH2PO4 buffer (pH 7.4) 2 mM GSH Prepared from 100 mM GSH 0.4 g Glycerol 2 mL of 0.5% γ-globulin Add 0.4 g of glycerol and 2 mL of 0.5% γ-globulin to 198 mL of 0.1 M K2HPO4/KH2PO4 buffer (pH7.4).
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.