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Assay Method Information

Assay Name:  Kinase Inhibition Assay
Description:  The enzyme was assayed with a biotinylated peptide substrate and test compounds in the presence of 10 uM ATP/[gamma-33P]ATP in a streptavidin coated FlashPlate (Perkin-Elmer, Boston, MA). Inhibition of the enzymatic activity due to the compound was measured by observing a reduced amount of 33P-gamma-ATP incorporated into the immobilized peptide relative to untreated controls. Linear regression analysis of the percent of inhibition by the test compound was used to calculate IC50 values.
Affinity data for this assay
 

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Last update November 1, 2007
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