new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  Inhibition of HIV protease was measured by assay of the cleavage of a fluorescent peptide substrate. The fluorescent product (2-aminobenzoyl-Ala-Thr-His-Gln-Val-Tyr) was separated from the fluorescent substrate by anion-exchange HPLC on a Mono-Q anion-exchange column (Pharmacia), and fluorescence was monitored at an excitation wavelength of 330 nm and an emission wavelength of 430 nm. Ki values for inhibitor binding were estimated at fixed concentrations of substrate and inhibitor from fractional activity measurements by use of the modified Michaelis-Menten equation.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.