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Assay Method Information

Assay Name:  ROS Production Measurement on hNOX1 Membranes
Description:  All solutions were placed on ice and protected from light. The final concentration in the 1x hNOX1 membrane fluorescent assay buffer was PBS pH7, 6 μM FAD, 15 μM PA, 1 mM MgCl2, 12.5 μM AR, 0.02 u/ml, 125 ng membranes, 1.5 μg of cofactors and 30 μM NADPH.The NADPH was dissolved in water at a concentration of 12 mM and was transferred in a metal transfer plate kept at 4 C. The NADPH was added to the assay plate to initiate the reaction just before the measurement.
Affinity data for this assay
 

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Last update November 1, 2007
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