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| | In vitro binding of inhibitor to hCA-II |
| Description: | Inhibitor binding to CA II was determined using a fluorescence competition assay. Displacement of dansylamide and binding of the inhibitor was determined by measuring and comparing the average fluorescence intensities of the treated versus control reactions. Binding constants were estimated from a Dixon plot of the data (reciprocal of the average fluorescence intensity versus inhibitor concentration) |
| Affinity data for this assay | |
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