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Assay Method Information

Assay Name:  PKA/PKC Kinase Assay
Description:  The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidin-coated FLASH plates. After reaction, the 33P-phosphopeptide captured on the FLASH plates was measured with a TopCount Packard Instruments gamma counter (Packard Instruments, Boston, MA). IC50s were calculated using the sigmoidal curve fit with the Assay Explorer software (MDL, Los Angeles, CA).
Affinity data for this assay
 

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Last update November 1, 2007
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