Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Kinase Selectivity Assay |
| Description: | Ser/Thr-kinase selectivity assays were performed using a radioactive FlashPlate-based assay platform. Substrate incorporated radioactivity was counted using a TopCount microplate reader (Perkin-Elmer). IC50 values were determined from six compound titration curves, and corresponding Ki values were calculated using the Cheng-Prusoff equation Ki = IC50/(1 + ([ATP]/Km)). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||