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Assay Method Information

Assay Name:  Androsterone Reduction Inhibition Assay
Description:  Enzyme activity was measured in the reductive direction against varying concentrations of androsterone. Initial velocities were measured by observing the rate of change of absorbance of pyridine nucleotide at 340 nm. Calculation of kcat and Km values used the Leonora program, yielding estimates of the kinetic constants. For inhibition studies, IMN and FLF were added at several different concentrations to give final concentrations varying between 0.5 and 50 uM, and steroid substrate concentrations were then varied as indicated above. The type of inhibition and the inhibition constants were calculated using the Leonora program. All reactions were initiated by the addition of enzyme.
Affinity data for this assay
 

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Last update November 1, 2007
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