Assay Method Information |
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| Radioligand Labeled Binding Assay |
Description: | Log IC50 values for each test compound were determined from nonlinear regression analysis of data collected from two independent experiments performed in duplicates (40 independent experimental values) using GraphPad Prizm 4 software (GraphPad, San Diego, California). The inhibition constant (Ki) was calculated from the antilogarithmic IC50 value by the Cheng and Prusoff equation. |
Affinity data for this assay | |
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