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| | CPA Inhibition Assay |
| Description: | Assay for the inhibition of carboxypeptidase A (CPDA) was conducted in 96-well microplates format. Activity was monitored by measuring decrease in absorbance (328 nm) over a 15 minute time course using a Spectromax 250 absorbance plate reader. Compound IC50 values were determined using 10-pt concentration ranges. Rates of substrate hydrolysis were determined from time courses and converted to percent inhibition. Percent inhibition was plotted versus compound concentration and fit to the four parameter logistic equation by non-linear regression using Prism Graphpad software. |
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