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Assay Method Information

Assay Name:  H3R Radioligand Binding Assay
Description:  Ligand displacement assays were performed on CHO cells membranes expressing hH3R. Retained radioactivity was determined by liquid scintillation counting. Nonspecific binding was determined in the presence of 1 uM thioperamide. The Ki values were calculated based on an experimentally determined appropriate Kd value according to Cheng and Prusoff.
Affinity data for this assay
 

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Last update November 1, 2007
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