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Assay Method Information |
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| | GAR Tfase Activity Assay |
| Description: | Assays were initiated by the addition of GAR to the reaction mixture containing GAR Tfase, test compounds, and cofactor. The assay monitors the deformylation of fDDF resulting from the transfer of the formyl group to GAR. Reaction rates were measured in triplicate using a Gilford 252 spectrophotometer. Double reciprocal plots were used to determine the Ki values. |
| Affinity data for this assay | |
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