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| | Protease Inhibition Assay |
| Description: | HIV-1 protease activity was measured by a continuous fluorometric assay using the internally quenched fluorogenic substrate DABCYL-GABA-Ser-Gln-Tyr-Pro-Ile-Val-Gln-EDANS (BACHEM; M-1865) and a SPEX FluoroMax spectrofluorometer (excitation 340 nm, emission 490 nm). The apparent Ki was estimated by nonlinear regression (GraFit, Erithacus Software) to the equation for tight-binding inhibitor. |
| Affinity data for this assay | |
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