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Assay Method Information

Assay Name:  Time Resolved Fluorescence Resonance Energy Transfer Assay
Description:  Lck activity was assessed using a TR-FRET assay in a 384-well plate format. The degree of phosphorylation of Biotinylated substrate was measured using a Packard Discovery plate reader as a ratio of specific 665 nm energy transfer signal to reference Eu 620 nm signal. All values quoted are means of IC50 results. Ki values were calculated from IC50s using Cheng-Prusoff equation.
Affinity data for this assay
 

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Last update November 1, 2007
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