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Assay Method Information |
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| | MMP Inhibition Assay |
| Description: | A continuous assay was used in which the substrate is a synthetic peptide containing a fluorescent group (7-methoxycoumarin), which is quenched by energy transfer to a 2,4-dinitrophenyl group. When the peptide was cleaved by MMPs, an increase in fluorescence was observed. The enzymatic reactions were initiated by adding the substrate, and the initial rate of the cleavage reaction was determined immediately after substrate addition. For most MMP assays in this study, the IC50 value is approximately 2-fold of the Ki value. |
| Affinity data for this assay | |
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