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Assay Method Information |
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| | NS5B Polymerase Inhibition Assay (IC50) |
| Description: | Assays were performed in a 96-well streptavidin-coated Flash-Plate using enzyme, RNA substrate, and [alpha-33P]GTP/GTP with inhibitor concentration varied over a suitable range. The reaction was stopped by aspiration after 75 min incubation and the plate was washed. After washing and drying the plate, incorporated radioactivity was counted using a Microbeta scintillation counter. IC50 values were calculated relative to the uninhibited control and inhibition data were fitted to a 4-parameter IC50 equation. The estimated average standard deviation for IC50 data is 32% from the mean value. |
| Affinity data for this assay | |
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