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Assay Method Information |
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| | GW0385 Assay |
| Description: | The assay method employed kinetic determinations of values for k1 and k-1, from which value of inhibition constant (Ki ) was determined (k-1/k1). The k1 is calculated from spectrofluorometric data in the presence of saquinavir. The k-1 is calculated from the time course for displacement of [3H]GW0385 from E[3H]GW0385 by nonlabeled GW0385. |
| Affinity data for this assay | |
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