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Assay Method Information

Assay Name:  PDGFR-beta Inhibition Assay
Description:  The autophosphorylation assay was performed in 96-well plates using the recombinant GST-fused kinase domains expressed in baculovirus and purified over glutathione-Sepharose. 33P-ATP was used as the phosphate donor, and the PDGFR-beta was used as the acceptor. IC50 values were calculated by linear regression analysis of the percentage inhibition of each compound in duplicate, at three concentrations (usually, 0.01, 0.1, and 1 uM or 0.1, 1, and 10 uM).
Affinity data for this assay
 

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Last update November 1, 2007
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