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Assay Method Information |
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| | Inhibition of Steady-State Catalysis Assay |
| Description: | To determine the IC50 value of each inhibitor, reactions were initiated by addition of 154 pM NQO2 to a reaction buffer containing 150 μM SCDP and 5 μM menadione with TBB (0.625−640 μM), TBBz (2.5−640 nM), or DMAT (2.5−640 nM). |
| Affinity data for this assay | |
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