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Assay Method Information

Assay Name:  HDAC Activity Assays
Description:  Purified HDAC1, HDAC2, and HDAC3 (in complex with the deacetylase activation domain of the human NCOR2 (amino acids 395 498)) were obtained from BPS Bioscience. The enzyme activities were initially tested in a serial dilution of each HDAC using the HDAC-Glo I/II reagents (Promega) according to the manufacturer s protocol. Luminescence was detected using the BMG POLARstar Omega microplate reader. A concentration of each HDAC within the linear response region was used for assaying inhibition of HDAC activity by UF010 and analogs. Each compound was tested in 10-point dose-response assay in triplicate.
Affinity data for this assay
 

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Last update November 1, 2007
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