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Assay Method Information

Assay Name:  HDAC Activity Assays
Description:  Purified HDAC1, HDAC2, and HDAC3 (in complex with the deacetylase activation domain of the human NCOR2 (amino acids 395┐498)) were obtained from BPS Bioscience. The enzyme activities were initially tested in a serial dilution of each HDAC using the HDAC-Glo I/II reagents (Promega) according to the manufacturer┐s protocol. Luminescence was detected using the BMG POLARstar Omega microplate reader. A concentration of each HDAC within the linear response region was used for assaying inhibition of HDAC activity by UF010 and analogs. Each compound was tested in 10-point dose-response assay in triplicate.
Affinity data for this assay
 

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Last update November 1, 2007
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