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Assay Method Information |
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| | Biochemical Assay (ATP at 50x Km) |
| Description: | Briefly, MtPanK activity was measured in a pyruvate kinaselactate dehydrogenase-coupled assay where the reaction rate was determined by spectrophotometric measurement of NADH consumption. A second set of IC50 measurements were performed with the ATP concentration at 50× Km, to determine the effect of cofactor competition. |
| Affinity data for this assay | |
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