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Assay Method Information |
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| | Isothermal Titration Calorimetry (ITC) |
| Description: | ITC experiments were performed by Auto ITC200 (Microcal) at 25 °C. ITC sample cell was filled with 10 μM PRC2 in titration buffer (25 mM HEPES pH 8.0, 150 mM NaCl, 1% DMSO). 40 μl of 100 μM EED226 in the same titration buffer was loaded in the ITC syringe. 19 injections, each 2 μl, from the syringe were injected into the ITC cell at 150 s intervals. For the EED226 titrating to EED protein, the cell was filled with 15 μM EED while 100 μM EED226 in the syringe. |
| Affinity data for this assay | |
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