Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | RNAse H Binding Assay |
| Description: | In this case, 1 μM HIV-1 RT was added to 50 mM Tris-HCl (pH 7.8), 50 mM NaCl, 6 mM MgCl2, and varying concentrations of GSK5750 to form enzyme-inhibitor (E-I) complexes. E-I complexes were rapidly mixed with 100 nM DNA/RNA hybrid (PBS-22dpol/PBS-50r) and 1 mM EDTA. Concentrations of GSK5750 were 0, 250, 500, 1000, 2000, 5000 nM. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||