Assay Method Information |
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| FXa Inhibition Assay (in prothrombinase) |
Description: | For comparison with the prothrombinase reaction the assays were also conducted with S2765 at 25 and 37°C in a similar buffer: 20 mM HEPES, pH 7.5 containing 0.1% PEG 8000, 5 mM CaCl2, 0.15 M NaCl, and 25 μM PC:PS phospholipid vesicles with or without 2 nM FVa. Final reagent concentrations are shown in parentheses. Apixaban (0 and 0.25-4.0 nM in 2-fold increments) and S2765 (31.25-1000 μM by 2-fold increments) were incubated for 10 min. Reactions at 37°C were initiated by addingFXa (50 pM). |
Affinity data for this assay | |
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