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Assay Method Information

Assay Name:  Glutathione S-transferase Activity Assay
Description:  Enzymatic activity was determined spectrophotometrically by measuring the conjugation of CDNB with GSH. The 1 ml assay mixture contained 0.5 mM CDNB, 1 mMGSH, and 100 mM potassium phosphate buffer, pH 6.5. The rate of increase in absorbance at 340 nm was measured for 5 mm at 37C against a blank containing thereaction mixture without enzyme.
Affinity data for this assay
 

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Last update November 1, 2007
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