Assay Method Information |
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| Inhibition Assay |
Description: | For selectivity studies, the PTPs, including LYP, mPTPA, SHP1-D1C, PTP1B, LMPTP, VHR, Laforin and PTPα-D1D2 were expressed and purified from E. coli. The inhibition assay for these PTPs were performed under the same conditions as SHP2 (D2C) except using a different pNPP concentration corresponding to the Km of the PTP studied. |
Affinity data for this assay | |
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