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Assay Method Information

Assay Name:  FAAH Inhibition Assay (30 min Preincubation)
Description:  FAAH activity was measured by following the production of ammonia generated from the hydrolysis of oleamide by FAAH. GDH catalyzes the condensation of ammonia and alpha-ketoglutarate to glutamate with a concomitant conversion of NADH to nicotinamide adenine dinucleotide, oxidized form (NAD+), which is spectrophotometrically measured at 340 nm. Test compounds were preincubated with FAAH at room temperature for 30 min. The reaction was initiated with the addition of substrate oleamide. The absorbance at 340 nm was collected over a period of 30 min with reading taken in 10-s intervals using a spectrophotometer.
Affinity data for this assay
 

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Last update November 1, 2007
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