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Assay Method Information |
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| | Surface Plasmon Resonance (SPR) |
| Description: | SPR studies were performed using a Biacore T200 (GE Health Sciences Inc.) at 20 °C. Approximately 8,400 response units (RU) of biotinylated EED was captured onto one flow cell of a streptavidin-conjugated SA chip (according to manufacturer's protocol) while another flow cell was left empty for reference subtraction. A-395 was dissolved in 100% DMSO (50 mM Stock) and diluted to 400 nM before triplicate two-fold serial dilutions were prepared in 100% DMSO. |
| Affinity data for this assay | |
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