| Details for Substrate
DNA Substrate without Explicit Binding Affinity Data |
| Binding Enzyme 1: | Genome polyprotein |
| Synonyms: |
n/a
|
| Type: | Duplex DNA with a single-stranded DNA overhang |
| Topology: | n/a |
| Mol. Mass.: | 2430.76 Dalton |
| Organism: | n/a |
| Description: | The substrate was prepared by annealing a 30mer oligonucleotide (template strand) to a 15mer (release strand). The resulting 15 bp duplex contained a single-stranded DNA overhang of 15 nt. A quencher BHQ1 was labeled at the 3 end of the release strand, and a fluorescein isothiocyanate (FITC) was labeled at the 5 end of the template strand. |
| Residue: | 30 |
| Sequence: | 5-CATCATGCAGGACAGTCGGATCGCAGTCAG-3 |
| |
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