Solution Information | help | |
Enzyme: | Cannabinoid receptor 2 | |
inhibitor: | BDBM76039 | |
substrate: | n/a | |
Solution Type: | Aqueous | |
pH at Preparation: | n/a | |
Temp. Prep.: | n/a | |
Comments: | Assay Materials: 1) 384-well plates, black with clear bottom (Aurora Biosciences # 032451) 2) U2OS (Human Osteosarcoma) cell line stably expressing the Beta-arrestin GFP and the CB2 receptor 3) Culture Media: MEM with L-glutamine, Pen-strep, 10% Fetal Bovine Serum and selection antibiotics - 200 ug/ml G418 and 100 ug/ml Zeocin 4) Positive Control Working Solution: WIN55212 (Sigma W102 25m g) - 5mM stock in DMSO). 5) Negative Control Working Solution: 100% DMSO 6) Test compounds from dry powders working solution: 10 mM in 100%DMSO 7) Fixative Working Solution: 6% Paraformaldehyde (PFA) diluted in PBS. 8) Nuclear Stain Working Solution: DAPI (Invitrogen, D1306) diluted to 150 ng/ml in DAPI buffer (10 mM TRIS, 10 mM EDTA, 100 mM NaCl, pH 7.4). Hit Confirmation Procedure: 1) 45 ul of cell suspension (200,000 cells/ml in culture medium) was dispensed in each well of the assay plates using a Wellmate bulk dispenser. 2) Plates are incubated overnight or approximately 20 hours at 37 degre | |
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |