Search and Browse
Download
Enter Data
| Solution Information | help | |
| Enzyme: | 2'-phosphotransferase | |
| inhibitor: | BDBM52921 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose-response curves for 20 compounds that demonstrated differential activities in a set of experiments entitled, "Fluorescence polarization-based confirmation biochemical high throughput screening assay to identify inhibitors of tRNA 2'-phosphotransferase (TPT1)" (AID 2149) and "Fluorescence polarization-based counterscreen assay for inhibitors of tRNA 2'-phosphotransferase (TPT1): biochemical high throughput screening assay to identify inhibitors of RNAse T1" (AID 2153). Compounds with a greater activity in the TPT1 assay compared to the RNase T1 counterscreen were selected and sorted by their distance (D) from a state where percent inhibition in both assays were equal according to the following equation: D = square root ( squared ( "% inhibition TPT1 assay" - "% inhibition RNase T1 counterscreen" ) / 2 ) The 20 compounds with the highest D value were selected for dose-response determinations. In this biochemical | |
| If you find an error in this entry please send us an E-mail | ||
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||