Reaction Details | |||
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Report a problem with these data | |||
Cell Reactant: | CAM-alpha-lactalbumin | ||
Syringe Reactant: | SecB | ||
Meas. Tech.: | ITC | ||
Entry Date: | 12/13/00 | ||
ΔG°: | -6.63± (kcal/mole) | ||
pH: | 7.4±n/a | ||
Log10Kb: | 4.96± n/a | ||
Temperature: | 293.15±n/a (K) | ||
ΔH° : | -5.43± (kcal/mole) | ||
ΔHobs: | n/a | ||
Ionic Strength: | n/a | ||
n/a | |||
Protons Released: | n/a | ||
ΔCp : | n/a | ||
Stoich. Param.: | 1 | ||
ΔS° : | 0± (kcal/mole-K) | ||
Comments: | discrepancies in the fluorescence and calorimetric-derived binding constants | ||
Citation | Panse, VG; Swaminathan, CP; Surolia, A; Varadarajan, R Thermodynamics of substrate binding to the chaperone SecB. Biochemistry39:2420-7 (2000) [PubMed] Article | ||
More Info.: | Get all data from this article , ITC RUN data , Solution Info , Instrument Info | ||
CAM-alpha-lactalbumin | |||
Source: | n/a | ||
Purity: | n/a | ||
Prep. Method: | Reduction with DTT and subsequent titration with DTNB, confirmed the number of disulfides present. | ||
Name: | CAM-alpha-lactalbumin | ||
Synonyms: | 2S-ScamLA | 3S-ScamLA | LALBA | LALBA_HUMAN | LYZL7 | ||
Type: | Protein | ||
Mol. Mass.: | 16219.69 | ||
Organism: | Homo sapiens (Human) | ||
Description: | P00709 | ||
Residue: | 142 | ||
Sequence: |
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SecB | |||
Source: | Expressed in E. coli strain BL21 (DE3) | ||
Purity: | 99% | ||
Prep. Method: | Fast flow Q-Sepharose column, and then a Sephacryl S-200 gel filtration column | ||
Name: | SecB | ||
Synonyms: | n/a | ||
Type: | Protein | ||
Mol. Mass.: | 17264.58 | ||
Organism: | Shigella flexneri serotype 5b (strain 8401) | ||
Description: | Q0SYD5 | ||
Residue: | 155 | ||
Sequence: |
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